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New Monograph: FRET Substrates

08. Nov 2012

The new product monograph 'FRET Substrates' gives you an overview of BachemĀ“s offer of FRET substrates and related products.

This revised edition has been enriched with clickable product deeplinks leading directly to the respective products in our Online Shop.


Fluorescence Resonance Energy Transfer (FRET) is the non-radiative transfer of energy from an excited fluorophore (or donor) to a suitable quencher (or acceptor) molecule. FRET is used in a variety of applications including the measurement of protease activity of substrates, with the fluorophore separated from the quencher by a short peptide sequence containing the enzyme cleavage site. Proteolysis of the peptide results in fluorescence as the fluorophore and quencher are separated.