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In This Issue
Bachem Peptide News Fragments **NEW**
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You can order the 2012 Bachem Immunology Catalog by mail or you can also search our online shop for immunology products including antibodies, antisera, EIA kits, and RIA kits.
Linda Haag is a Master Scheduler for Instrumentation Laboratory's reagent manufacturing facility in Orangeburg, NY. Instrumentation Laboratory builds quality diagnostic laboratory equipment for critical care analysis. Bachem's BOC amino acids are used in the formulation of many of Instrumentation Laboratory's chromogenic substrates.
As a prize, Linda received a USD $25 VISA gift card.
See this month's Amino Acid Sudoku for your chance to win this month's prize: a Bachem Swiss Army Knife.
Metallothionein, a Heavy Metal Binding Protein
Dr. Roger Acey's lab at California State University (CSU) Long Beach is interested in the biochemical and genetic factors controlling early embryonic development. They are using the brine shrimp Artemia salina ("the sea monkeys" found in the back of old comic books) as a model organism. In particular, they are studying how Artemia maintains intracellular trace metal homeostasis and the role of trace metals in regulating gene transcription.
To this end, Acey's group has cloned the gene encoding a metallothionein-like protein. Metallothionein has the unique ability to selectively bind both toxic and precious heavy metals, e.g., mercury, lead, gold, and platinum. It does not bind essential trace metals such as calcium and magnesium. Metal binding occurs instantaneously on contact with dissociation constants greater than 10-12. The protein will bind metal at extremes of pH (4 to 10) and temperature (4 to 100°C).
Regulation of Intracellular Trace Metal Concentrations by Metallothionein
Dr. Acey's lab is studying metallothionein as a "metal buffer" to regulate intracellular concentrations of essential metals such as zinc. Zinc is important for the function many enzymes; for example, zinc is critical for function of the gene transcription apparatus RNA Polymerase II. As such, changing the bioavailability of zinc can also change activity of RNA Polymerase II and ultimately regulate gene expression.
Recent studies in particular support the concept that metallothionein is a "storage depot" for essential trace metals such as zinc. It has been seen that metallothionein can directly transfer metals to apo-zinc metalloproteins. Dr. Acey's lab is continuing to study how metallothionein can affect gene expression, particularly during embryonic development of Artemia.
Environmental Applications of Metallothionein
The unique metal binding properties of metallothionein led Dr. Acey to develop a technology using the protein as a "heavy metal sponge" to remove toxic or precious metals from solutions. The device can also be adapted to remove mercury based preservatives from pharmaceuticals or to clean up toxic waste sites.
About Dr. Acey
Dr. Roger Acey is professor of Biochemistry at CSU Long Beach. He is the holder of four US and three international patents on the technology described here. There are also ten US/International patents pending.
To learn more about Dr. Acey's research, visit his website at
To read publicly available information on Dr. Acey's patents, visit Google Patent Search.
Contact Dr. Acey by email at email@example.com.
We scour the Internet and share with you news about peptides in basic research and pharmaceutical development. These are Bachem Peptide News Fragments.
BYETTA® Approved for Use with Basal Insulin in Europe
PEPTIDE RESEARCH NEWS
Frog skin protein may help fight superbugs—A few species of frogs are known to secrete peptides that protect against a broad range of unwanted bacteria.
Synthetic spider venom could add bite to crop protection—Researchers are looking at whether it is possible to mimic the insecticidal peptides found in spider venom compounds for use in controlling insects that threaten crops.
£140,000 for research into peptide nanotubes—Professor Dek Woolfson, Dr David Fermin, and Dr Franziska Thomas in the School of Chemistry have secured a three-year Leverhulme Trust Research Project Grant totaling £140,000. The award is to develop the design, assembly and functionalisation of peptide nanotubes, leading to nanothin metal wires.
Isabelle Sutter is an Inside Sales Representative responsible for the sales of our catalog products in France, the French-speaking part of Switzerland, Spain, Italy and Israel. She speaks and writes English, French, Spanish, and German fluently.
Isabelle joined Bachem in 2005 as a trainee, and has been an Inside Sales Representative since January 2007. In her leisure time, Isabelle likes reading lots of books, practicing her language skills while traveling through Europe, snorkeling, baking cakes, and cooking in general.
Bachem manufactures active pharmaceutical ingredients (APIs) for use throughout the world in investigational and commercial drug products. Bachem has the knowledge and experience necessary to meet the regulatory filing requirements of our customers from drug development through commercialization.
Bachem has 65 national EDMF approvals, 35 active US DMFs, 15 CEPs, and several active DMFs in many other countries. We have submitted many technical packages in support of successful IMPDs and INDs.
To ask Bachem a regulatory question, email us at firstname.lastname@example.org or click on the picture. Your question may be answered in a future issue of Bachem PEPTalk.
This Month's Peptide: AQEE-30 (human)
AQEE-30 is one of the fragments of the neurosecretory protein VGF. VGF is thought to be involved in the regulation of cell-cell interactions or in synaptogenesis during the maturation of the nervous system.
The VGF gene was originally identified in nerve growth factor-treated PC12 cells. It is expressed in a subset of neurons in the central and peripheral nervous systems and in specific populations of endocrine cells in the adenohypophysis, adrenal medulla, gastrointestinal tract, and pancreas. Proteolytic processing yields a number of peptides with activities in anti-depression, stress response, food intake, and energy homeostasis. For example, the mouse/rat AQEE-30 Pro-VGF-derived peptide (H-7276) has been shown to increase synaptic charge in hippocampal neurons and to evoke thermal hyperalgesia when injected intrathecally into naive adult mice.
Note: a second Leu has been replaced with Xxx.
Bachem offers various catalog peptides and immunology products for studying the neurosecretory protein VGF.
Each amino acid in the sequence listed above appears once in each of the nine rows, once in each of the nine columns, and once in each of the 3x3 boxes outlined with a double border.
Email your answers to email@example.com. Please submit correct entries before 12:01am Pacific Time, Monday, April 23, 2012.
One randomly-selected winner will receive a Bachem Swiss Army Knife.