Europe/Asia USA
Bachem AG Bachem Americas, Inc.
Hauptstrasse 144 3132 Kashiwa Street
4416 Bubendorf - Switzerland Torrance, CA 90505 - USA
Tel +41 61 935 2323 Tel +1 888 422 2436
Fax +41 61 935 2325 +1 888 4BACHEM
E-mail sales.ch@bachem.com +1 310 539 4171
  Fax +1 310 539 9428
  E-mail sales.us@bachem.com

GLOSSARY & ABBREVIATIONS

FRET

Fluorescence Resonance Energy Transfer

back to overview

Fluorescence Resonance Energy Transfer (FRET), the non-radiative transfer of energy from an excited fluorophor (donor) to a suitable acceptor (quencher). The transfer is influenced by the distance, the dipolar orientation, and the spectral overlap of the donor and acceptor moieties. In FRET substrates, fluorophor and quencher are linked tightly by a peptide chain. Enzymatic cleavage is accompanied by increasing fluorescence, as donor and acceptor are separated.

back to overview