Webinar 2D LC/MS: Peak purity determination for denaturing SAX oligonucleotide methods

Oligonucleotides are emerging new therapeutic entities and reliable selective analytical methods to determine their purity and related impurities are critical. Strong anion exchange (SAX) liquid chromatography (LC) methods are often used as an orthogonal method to well established ion-pairing reversed phase (IP-RP) methods, particularly for phosphorothioate oligonucleotides. However, the non-volatile eluents of SAX methods deem them not MS-compatible and are hindering direct peak purity analysis.

In this webinar, a novel 2D-LC-MS approach developed at Bachem will be described that allows the evaluation of peak purity by using SAX methods in combination with IP-RP and high-resolution MS.