## How to Calculate the Peptide Content?

#### Calculate the Peptide Content

The peptide content is the amount of pure peptide (net weight) in a product and a lot specific value, because it depends on the purity and assay (content) of the lot.

The peptide calculator uses the analytical data which are documented in the respective Analytical data sheet (ADS) for Bachem’s products as shown in this example.

Please enter the values for purity (%), assay (%) and the weight (mg) of the sample and the net weight will be calculated*.

#### BACHEM’S PEPTIDE CONTENT CALCULATOR

The following formula is used for the calculation of the net weight:

For the calculation of Volume, Concentration or Gross Weight, Purity and Assay are taken into consideration, unless the checkbox is set to “Use 100% for Purity and Assay.”

The calculation of the solvent volume is based on the following equation:

The result of this calculation is how much solvent is needed to dissolve a given quantity of lyophilizate to obtain a given concentration of pure peptide in solution.

The calculation of the concentration is based on the following equation:

The result of this calculation is what the concentration of pure peptide in solution will be, if a given quantity of lyophilizate is dissolved in a given volume of solvent.

The calculation of the gross weight is based on the following equation:

The result of this calculation is how much lyophilizate is needed to obtain a given amount of a solution, which has a given concentration of the pure peptide.

## Peptide Content Calculator

## Peptide Content Calculator

*For details on gross and net weight, please consult our Frequently Asked Questions.

** I.E. Choose “Volume” to calculate how much solvent you need to prepare a solution of a specific concentration with a given quantity of lyophilized peptide.

## Now that you have your results

Be sure to check our technical note Care and Handling of Peptides, which gives you detailed information on how to best prepare solutions of your Bachem product.

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