High-resolution mass spectrometry of oligonucleotides – characterization made easy

In this webinar we will present direct, robust and easy-to-use techniques to fully characterize the nucleotide sequence of highly modified single-stranded RNAs by top down tandem MS. This will include a case study for a N-acetylgalactosamine modified RNA sense strand. The approach can be applied on a single strand level, as well as on siRNA duplex level in a single electrospray ionization MS (ESI-MS) experiment. This procedure also allows us to reliably predict the structure of impurities that can occur during oligonucleotide production, which supports process improvements in manufacturing.

 

About the Speakers

Giovanni Calderisi

Project Chemist QC at Bachem AG

Giovanni joined Bachem in Bubendorf (Switzerland) in September 2020 as an expert in mass spectrometry of oligonucleotides. In his current position as a project chemist he is responsible for establishing routine workflows for characterization of oligonucleotides and peptides by ultra-high resolution mass spectrometry. Giovanni holds a Ph.D. in chemistry with focus on biomolecular mass spectrometry at the University of Innsbruck, Austria.

Seamus White

Senior Business Development Manager at Bachem AG

Seamus White is a Business Development Manager at Bachem Bubendorf. He is located in Northern Ireland and is the primary contact for our partners in the UK and Ireland in the area of GMP peptide API manufacture and development. In his role, he supports our customers through all phases of their project – from preclinical research and IND enabling toxicology studies to process validation and commercialization.

Seamus has been with Bachem since 2020, and prior to this he held positions in Global Procurement, Supplier Management, Lead Auditor and also has API Development and Manufacturing experience with a major Pharma. He has a Bachelor’s degree in Chemistry with Pharmacology and is a chartered Procurement Professional.

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